MY SKILLS

ENVIRONMENTAL CHEMISTRY

1) Characterization of Dissolved Organic Carbon using solid phase extraction and LC-MS techniques. Data processing by Progenesis and PetroOrg softwares.
2) Determine Total and Dissolved Organic Carbon concentrations in seawater (TOC/DOC) using a TOC analyser.
3) Analysis of metals in sediment by sequential extraction procedure (BCR method) and data interpretation from ICP-MS
4) pH spectrophotometric
5) Total Alkalinity using a potentiometric method
6) Estimation of inorganic carbon speciation in seawater

MARINE MICROBIOLOGY

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1) DNA extraction

2) Fluorescence in situ hybridization (FISH) in marine sediments

3) DAPI-stained using epifluorescence microscopy

4) Metabolic activity of marine bacteria  by colorimetric assay using tetrazodium salt (INT,  2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride) 

5) Respiring activity of marine bacteria using tetrazodium salt (CTC, 5-Cyano-2,3-ditolyl tetrazolium chloride (CTC) by epifluorescence microscopy

6) Determination of extracellular Polysaccharides Substances (EPS) 

ECOTOXICOLOGY

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1) Toxicity test using amphipods

2) Microtox ®

3) In vitro fertilization and embryonic development bioassays